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#Law Of Segregation - Mendel Pea Plant Experiment Mendel Pea Plant ExperimentShare this page on your website: Mendel Pea Plant ExperimentThe Law of Segregation is based on one of the benchmark scientific experiments in genetic studies, the Mendel Pea Plant Experiment. Save this course for laterIt was a stroke of genius considering that it was performed in the 19th century. Of course, Mendel knew nothing about the processes behind inherited behavior, but found out the rules dictating how characteristics are passed down through the generations. The way in which he meticulously planned his experiment to generate testable. rather than observational data, is a shining example of experimental design . Gregory Mendel was an Austrian monk studying at the University of Vienna. Like many pioneering scientists, his discoveries were largely ignored until long after his death. Mendel's Initial ObservationsMendel noticed that certain characteristics appeared to be passed on from parents to offspring, in many species, and wondered why this was so. He also wanted to establish what characteristics were taken from each parent. For the Mendel Pea Experiment he chose pea plants, because they had some measurable characteristics as well as being easy to breed and with a relatively short breeding period allowing a quick experimental turn-around. He measured seven of these characteristics, including seed-shape, seed color, pod-color and pod-shape, each occurring in two distinctive forms. MethodTo conduct the experiment. he cross pollinated the selected pea plants by removing the anthers from one flower, to prevent self-fertilization, and dusting pollen from another plant onto the flower. Initially, he cross-fertilized plants with the same characteristics to ensure that the plants were true-breeding, giving a good baseline for the research. The next stage was to cross-pollinate plants with different characteristics, such as one with green seed pods to one with yellow seed pods. These initial plants are called the P (Parental) generation. The resulting offspring, the F1 (Filial generation) were then self pollinated to produce the F2 generation. This self-fertilization continued until the traits were established for many generations. Mendel's Law of SegregationIn the case of pod color, the Mendel Pea Experiment showed that a cross between a green pod plant and a yellow pod plant produced only green pod plants for the F1 generation. It appeared that the yellow pod characteristic had disappeared. However, the F2 generation threw up a surprising result; the yellow pod variant appeared in a quarter of this generation. Clearly, some thing strange was going on and, in an inspired piece of thinking, Mendel came up with his 'Law of Segregation'.
The Mendel Pea Experiment and the discovery of the Law of Segregation has shaped the way that genetic research has developed and it has been shown that this law applies to all sexually reproducing organisms. Modern TerminologyModern geneticists always express genes as letters and numbers, with a dominant gene being a capital letter, a recessive gene a lowercase letter. For example, the Green pod gene is 'G', the yellow gene 'g'. Discussion - What is HappeningThe F1 generation all had the Gg combination and so were all green pod variants although, importantly, all had a 50% chance of passing on the recessive g allele to the next generation. The best way to express what happens in the F2 generation is with a type of diagram known as a Punnett Square. (Fig 1) As the Punnett Square shows, offspring with a dominant G gene will always have green pods with only the offspring containing the gg combination having yellow pods. This is why Mendel only found that one quarter of the F2 generation had yellow pods. However, all but one quarter had at least one 'g' allele and so this would continue to be passed down through the generations. Figure 1 - Law of Segregation
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